primary human lung smooth muscle cells  (ATCC)

Journal: Cell Death Discovery

Article Title: Trifluoperazine causes mast cell apoptosis through a secretory granule-mediated pathway

doi: 10.1038/s41420-026-03122-x

Figure Lengend Snippet: A Human lung smooth muscle cells (HLSMCs), lung fibroblasts (HLFs), and small airway epithelial cells (HSAECs) were treated with TFP at the indicated concentrations for 24 h. Human peripheral blood eosinophils and neutrophils were treated with TFP for 2 h. Cell viability was assessed by staining the cells with Annexin V (AnnV) and DRAQ7. Viable cells, AnnV − DRAQ7 − ; apoptotic cells, AnnV + DRAQ7 − ; necrotic/late apoptotic cells, AnnV + DRAQ7 + . HSAECs, n = 6 from three independent experiments; HLSMCs, eosinophils, neutrophils, n = 4 from four independent experiments; HLFs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA for HLSMCs, HLFs; Kruskal-Wallis for HSAECs; Friedman test for eosinophils, neutrophils). B – C Bone marrow-derived MCs (BMMCs) and peritoneal cell-derived MCs (PCMCs) treated under the same conditions as in ( A ) for 24 h. BMMCs, n = 5 from two independent experiments; PCMCs, n = 3 from one individual experiment representative of three independent experiments (One-way ANOVA). Untreated (control) cells were used for statistical comparisons to all other groups in all figures. The bar charts show mean values + SEM or median + interquartile range. * P < 0.05; ** P < 0 .01; **** P < 0.0001. D Effect of TFP on DNA degradation. MCs were preincubated with bafilomycin A1 (Baf A1) (20 nM) for 2 h followed by treatment with TFP (10 μΜ) for 2 h. DNA was extracted from MCs and fragmentation was assessed by agarose gel electrophoresis. St standard marker.

Article Snippet: Primary human lung fibroblasts (HLFs) (PCS-201-013) and primary human lung smooth muscle cells (HLSMCs)(PCS-130-010) were obtained from American Type Culture Collection (ATCC, Manassas, VA) and cultured following the manufacturer’s instructions.

Techniques: Staining, Derivative Assay, Control, Agarose Gel Electrophoresis, Marker

Journal: Nature Communications

Article Title: A vascularized liver microphysiological system captures key features of hepatic insulin resistance and monocyte infiltration

doi: 10.1038/s41467-025-68031-6

Figure Lengend Snippet: A Schematic of culture platform and seeding strategy, beginning with multicellular hepatic spheroid generation in alginate microwells for 2 days, followed by harvesting and co-encapsulation of spheroids, endothelial cells, and supporting fibroblasts inside of a fibrin hydrogel within polydimethylsiloxane (PDMS) microfluidic devices. Created in BioRender. Tevonian, E. ( https://BioRender.com/o38c467 ). B Self-organized vascular network formation after 6 days in devices. Maximum intensity projection of live image taken at 10x magnification shows GFP-HUVEC networks (green) interacting with hepatocytes labeled with CellTracker Deep Red (magenta). Inset (yellow box) shows a magnified view, with arrows indicating regions where networks are in close proximity with spheroids. Scale bars = 200 µm. This experiment with CellTracker-labeled spheroids was repeated independently seven times with similar results. C Live image taken on Day 4 after device seeding shows RFP-HUVECs (false-colored cyan) migrating out of spheroids to connect with GFP-HUVECs (green), with hepatocytes labeled with CellTracker Deep Red (magenta). Scale bar = 200 µm. This experiment with RFP-HUVECs was repeated independently nine times with similar results. ( D ) Representative maximum intensity projection image of an immunostained device after 14 days of culture. Arginase-1 (magenta) indicates hepatocytes, CD31 (green) indicates HUVECs, and CD163 (white) indicates Kupffer cells. Scale bars = 200 µm. This staining was repeated for devices from three unique primary liver cell donors with similar results.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; Angioproteomie, cAP-0001) and normal human lung fibroblasts (NHLFs; Lonza, CC-2512) were cultured in flasks using VascuLife VEGF Endothelial Medium Complete Kit (VascuLife; LifeLine, LL-0003) and FibroLife S2 Fibroblast Medium Complete Kit (FibroLife; Lifeline, LL-0011), respectively.

Techniques: Encapsulation, Labeling, Staining

Journal: Microbial Cell Factories

Article Title: Recombinant L-asparaginase from Stenotrophomonas maltophilia : a promising low-immunogenic anticancer agent

doi: 10.1186/s12934-025-02856-0

Figure Lengend Snippet: Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

Article Snippet: Human leukemia cancer cell line K-562, human hepatocellular carcinoma cell line HepG-2, and normal human lung fibroblast cell line MRC-5 were purchased from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Activity Assay, Recombinant, Produced, Standard Deviation